Tumor Research
[Abstract]
Objective To detect the expression of ATPaseF1?in colorectal cancer(CRC) and discuss its relationship with clinical and pathological parameters. To investigate the gene of ATPaseF1?in LoVo human colon cell line and the expression of ATPaseF1?on the cell surface membrane. To research the suppressing effect of anti-ATPaseF1?antibody on reproductive activity and metastasis activity of LoVo cell line. Methods The expression level of ATPaseF1?was examined by Envision and RT-PCR. The expression of ATPaseF1?on the cell surface of LoVo cell line was observed by immunocytochemistry and immunofluorescence. The suppressing effect of anti-ATPaseF1?antibody on reproductive activity of LoVo is evaluated by CCK-8 assay, and the similar effect of antibody on metastasis activity of LoVo is surveyed by Wound-healing assay. Results Immunocytochemistry analyses showed a significant increase in the expression of ATPaseF1?in CRC compared with adjacent normal colorectal tissue and benign samples. Positive ATPase F1?mRNA expression was confirmed by RT-PCR in LoVo cell line. The positive expression of ATPaseF1?on the cell surface of LoVo was observed.ATPaseF1?mAb showed depressant effect on cell proliferation. ATPaseF1?mAb obviously restrains the migration of LoVo cells.Conclusions Our overall findings demonstrate that as a novel tumor associated antigen, ATPaseF1?maybe will turn out a new way to targeted therapy of CRC.
Title: The Initial Research of ATPaseF1? Expression in Colorectal Cancer and Its Novel Biology Function
Category: Wilms Tumor
Filename: The Initial Research of ATPaseF1? Expression in Colorectal Cancer and Its Novel Biology Function.pdf
Pages: 189
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